The role of antibodies specific for toxic sPLA₂s and haemorrhagins in neutralizing potential of antisera raised against Vipera ammodytes ammodytes venom

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• 作者：Beata Halassy ; Lidija Habjanec ; Marija Brgles ; Maja Lang Balija ; Adrijana Leonardi ; Lidija Kovač ; ič ; Petra Prijatelj ; Jelka Tomaš ; ić ; Igor Križ ; aj
• 关键词：Vipera ammodytes ammodytes ; Phospholipase A₂ ; Ammodytoxin ; Haemorrhagin ; Antivenom ; ELISA ; Toxicity-neutralization potency
• 刊名：Comparative Biochemistry and Physiology, Part C
• 出版年：2008
• 出版时间：August 2008
• 年：2008
• 卷：148
• 期：2
• 页码：178-183
• 全文大小：425 K

文摘

The contribution of antibodies directed against the two main toxic groups of proteins in the Vipera ammodytes ammodytes venom, haemorrhagic metalloproteinases (H) and neurotoxic sPLA₂s (Atxs), to the overall protective efficacy of the whole venom antisera was investigated. Using ELISA assays we established a high correlation between the protective efficacy of the whole venom antisera in mice and their anti-Atxs antibody content. As the haemorrhage is the prevailing toxic effect of the venom in human, the lack of correlation also with anti-H IgG content exposed that the mouse model might not be optimal to evaluate the neutralizing potential of the venom-specific antisera for human therapy. We further revealed that Atxs and structurally very similar but non-toxic AtnI₂ from the venom are not immuno cross-reactive.