ISSR variations in eggplant (Solanum melongena

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• 作者：Shiro Isshiki ; Naoko Iwata ; Md. Mizanur Rahim Khan
• 关键词：ISSR ; Eggplant ; Solanum species ; Phylogenetic relationships ; Cultivar identification
• 刊名：Scientia Horticulturae
• 出版年：2008
• 出版时间：23 July 2008
• 年：2008
• 卷：117
• 期：3
• 页码：186-190
• 全文大小：353 K

文摘

Inter-simple sequence repeat (ISSR) analysis was performed in eight cultivars of eggplant (Solanum melongena) and 12 accessions in eight related Solanum species to evaluate the applicability of this analysis for assessing the phylogenetic relationships and identifying cultivars. A total of 552 polymorphic amplified bands were obtained from 34 of the 100 primers tested, and the percentage of polymorphisms was 99.1 % . Cluster analysis based on the ISSR markers classified the Solanum species into seven groups: (i) S. melongena; (ii) S. aethiopicum and S. anguivi; (iii) S. incanum; (iv) S. violaceum and S. kurzii; (v) S. macrocarpon; (vi) S. virginianum and (vii) S. torvum. Combining the ISSR markers obtained by a few of the 34 primers was enough for distinguishing of the eight cultivars of eggplant. This ISSR analysis was demonstrated to be available for the phylogenetic study and the cultivar identification.