Characterization of an alkaline β-agarase from Stenotrophomonas sp. NTa and the enzymatic hydrolysates
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- 作者：Yanbing Zhua ; b ; c ; d ; Rui Zhaoa ; Anfeng Xiaoa ; b ; c ; d ; Lijun Lia ; b ; c ; d ; Zedong Jianga ; b ; c ; d ; Feng Chena ; e ; Hui Nia ; b ; c ; d ; nihui@jmu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：SDS-PAGE ; sodium dodecyl sulfate-polyacrylamide gel electrophoresis ; DNS ; 3 ; 5-dinitrosalicylic acid ; MALDI-TOF MS ; matrix-assisted laser desorption ionization-time of flight mass spectrometer ; EDTA ; ethylenediaminetetraacetic acid ; β-ME ; β-mercaptoethanol ; DTT ; dithiothreitol ; PMSF ; phenylmethylsulfonyl fluoride ; SDS ; sodium dodecyl sulfate ; VC ; vitamine C ; DPPH ; 2 ; 2-diphenyl-1-picryl-hydrazyl-hydrate ; BHTxps ; butylated hydroxytoluene ; ABTS ; 2 ; 2&prime ; -azino-bis (3-ethylbenzothiazoline-6-sulfoni
- 刊名：International Journal of Biological Macromolecules
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：86
- 期：Complete
- 页码：525-534
- 全文大小：1636 K
文摘
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This is the first to present evidence of agarolytic activity in strain from genus Stenotrophomonas. We purified an extracellular β-agarase from marine bacterium Stenotrophomonas sp. NTa. This alkaline agarase exhibited stricking stability across a wide pH range. It also had a relatively good resistance against some detected inhibitors, detergents and urea denaturant. We identified the enzymatic hydrolysates and their antioxidant activities.