Overexpression and secretion of AgaA7 from Pseudoalteromonas hodoensis sp. nov in Bacillus subtilis for the depolymerization of agarose
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- 作者：Kristine Rose M. Ramosa ; 1 ; Kris Niñ ; o G. Valdehuesaa ; 1 ; Rhudith B. Cabulonga ; Llewelyn S. Morona ; Grace M. Nisolaa ; Soon-Kwang Hongb ; Won-Keun Leeb ; wklee@mju.ac.kr" class="auth_mail" title="E-mail the corresponding author ; Wook-Jin Chunga ; wjc0828@gmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Agarase ; Protein secretion ; Signal peptide
- 刊名：Enzyme and Microbial Technology
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：90
- 期：Complete
- 页码：19-25
- 全文大小：1545 K
文摘
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An extracellular β-agarase gene (agaA7) was cloned in B. subtilis.
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The recombinant B. subtilis was able to secrete a functional AgaA7.
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Signal peptide screening was performed to optimize secretion of AgaA7.