Immobilized enzyme mediated synthesis of silver nanoparticles using cross-linked enzyme aggregates (CLEAs) of NADH-dependent nitrate reductase
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- 作者：Sachin Talekar ; sachintalekar7@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Asavari Joshi ; Radhika Chougle ; Arya Nakhe ; Rahul Bhojwani
- 关键词：Silver nanoparticles ; Nitrate reductase ; Cross-linked enzyme aggregates (CLEAs) ; Enzyme immobilization
- 刊名：Nano-Structures & Nano-Objects
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：6
- 期：Complete
- 页码：23-33
- 全文大小：3084 K
文摘
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We synthesize silver nanoparticles using immobilized nitrate reductase as CLEAs.
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Effect of various process parameters on silver nanoparticle synthesis was studied.
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Spherical fcc silver nanocrystals of 5–7 nm diameter were formed.
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Nitrate reductase CLEAs were reused for repetitive synthesis in batch mode.
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Silver nanocrystals of same size and shape were formed in each batch reaction cycle.