A novel cross-linked enzyme aggregates (CLEAs) of papain and neutrase-production, partial characterization and application

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• 作者：Zhongqin Chen ; Yanwei Wang ; Wei Liu ; Jingya Wang ; Haixia Chen ; ^{chenhx@tju.edu.cn}
• 关键词：Papain ; Neutrase ; Cross-linked enzyme aggregates (CLEAs) ; Characterization ; Protein hydrolysis
• 刊名：International Journal of Biological Macromolecules
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：95
• 期：Complete
• 页码：650-657
• 全文大小：2143 K
• 卷排序：95

文摘

A novel Cross-linked enzyme aggregates (CLEAs) of papain and neutrase have been prepared. Cross-linking conditions of P-N-CLEAs have been optimized to achieve high enzyme activity. Cross-linking improves stability of the enzyme papain and neutrase. P-N-CLEAs had good application in both water and ethanol soluble protein hydrolysis.