Formation of cross-linked nitrile hydratase aggregates in the pores of tannic-acid-templated magnetic mesoporous silica: Characterization and catalytic application

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• 作者：Jing Gao^a ; ^b ; Hao Yu^a ; ^b ; Liya Zhou^a ; ^b ; Ying He^a ; ^b ; Li Ma^a ; ^b ; Yanjun Jiang^a ; ^b ; ^{yanjunjiang@hebut.edu.cn}
• 关键词：Enzyme biocatalysis ; Nitrile hydratase ; Tannic acid ; Magnetic mesoporous silica nanoparticles ; Immobilized enzyme ; Nicotinamide
• 刊名：Biochemical Engineering Journal
• 出版年：2017
• 出版时间：15 January 2017
• 年：2017
• 卷：117
• 期：part_PA
• 页码：92-101
• 全文大小：2549 K
• 卷排序：117

文摘

Tannic-acid-templated magnetic mesoporous silica nanoparticles are synthesized. Immobilized NHase is prepared by adsorption and cross-linking method. Immobilized NHase exhibits improved thermal, pH, mechanical and storage stability. Yield of nicotinamide can reach more than 98% by using immobilized NHase as catalyst.