Neurochemistry of myenteric plexus neurons of bank vole (Myodes glareolus) ileum
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- 作者：J. Sadeghinezhad ; C. Sorteni ; G. Di Guardo ; C. D鈥橝gostino ; U. Agrimi ; R. Nonno ; R. Chiocchetti
- 关键词：Enteric nervous system ; Immunohistochemistry ; Neurochemical code ; Bank vole
- 刊名：Research in Veterinary Science
- 出版年：December, 2013
- 年：2013
- 卷：95
- 期：3
- 页码：846-853
- 全文大小：1040 K
文摘
The neurochemistry of enteric neurons differs among species of small laboratory rodents (guinea-pig, mouse, rat). In this study we characterized the phenotype of ileal myenteric plexus (MP) neuronal cells and fibers of the bank vole (Myodes glareolus), a common rodent living in Europe and in Northern Asia which is also employed in prion experimental transmission studies. Six neuronal markers were tested: choline acetyltransferase (ChAT), neuronal nitric oxide synthase (nNOS), calbindin (CALB), calcitonin gene-related peptide (CGRP) and substance P (SP), along with HuC/D as a pan-neuronal marker.
Neurons expressing ChAT- and nNOS-immunoreactivity (IR) were 36 卤 12% and 24 卤 5%, respectively. Those expressing CGRP-, SP- and CALB-IR were 3 卤 3%, 21 卤 5% and 6 卤 2%, respectively. Therefore, bank vole MPs differ consistently from murine MPs in neurons expressing CGRP-, SP- and CALB-IR.
These data may contribute to define the prion susceptibility of neuron cell populations residing within ileal MPs from bank voles, along with their morpho-functional alterations following oral experimental prion challenge.