Transcription factors regulating uspA genes in Catharanthus roseus

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• 作者：Ahmed Bahieldin^a ; ^b ; ^{bahieldin55@gmail.com} ; Ahmed Atef^a ; ^{ahmed_atefaig2@yahoo.com} ; Ahmed M. Shokry^a ; ^c ; ^{ahmedshokry1@gmail.com} ; Saleh Al-Karim^a ; ^{Skarim@kau.edu.sa} ; Sanaa G. Al Attas^a ; ^{sangtas@mit.edu} ; Nour O. Gadallah^a ; ^d ; ^{nouromar71@yahoo.com} ; Sherif Edris^a ; ^b ; ^e ; ^{sedris@aucegypt.edu} ; Magdy A. Al-Kordy^a ; ^d ; Sabah M. Hassan^a ; ^b ; ^{m_alkordy@yahoo.com} ; Salah Abo-Aba^a ; ^f ; ^{sabmahmoud@yahoo.com} ; Fotouh M. El-Domyati^a ; ^b ; ^{fm_domyati@hotmail.com}
• 关键词：Transcription factor ; Co-expression ; Erf ; BHLH ; WRKY ; RNA-Seq ; SqRT-PCR
• 刊名：Comptes Rendus Biologies
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：340
• 期：1
• 页码：1-6
• 全文大小：1252 K
• 卷排序：340

文摘

RNA-Seq of the Catharanthus roseus SRA database was done in order to detect putative universal stress proteins (USPs) and their possible controlling factors. Previous analysis indicated the existence and characterization of uspA-like genes. In silico analysis of RNA-Seq database in several plant tissues revealed the possible functions and regulations of some uspA-like transcripts whose transcription factors (TFs) that might drive their expression were detected. BLAST indicated the existence of TF superfamilies erf (ethylene-responsive TF), bHLH (basic helix-loop-helix) and WRKY that might regulate several uspA-like genes. This data was proven via semi-quantitative RT-PCR in four plant tissues. Several of these transcription factor superfamilies are known for their action in the plant defense against biotic and abiotic stresses.