The full-length clone of cucumber green mottle mosaic virus and its application as an expression system for Hepatitis B surface antigen
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- 作者：AikSeng Ooi ; SiangHee Tan ; Rosmawati Mohamed ; Noorsaadah Abdul Rahman and Rofina Yasmin Othman
- 关键词：CGMMV ; Infectious transcripts ; HBsAg ; Tobamovirus ; Chimeric virus
- 刊名：Journal of Biotechnology
- 出版年：2006
- 出版时间：24 February 2006
- 年：2006
- 卷：121
- 期：4
- 页码：471-481
- 全文大小：487 K
文摘
A cucumber green mosaic mottle virus (CGMMV) full-length clone was developed for the expression of Hepatitis B surface antigen (HBsAg). The expression of the surface displayed HBsAg by the chimeric virus was confirmed through a double antibody sandwich ELISA. Assessment of the coat protein composition of the chimeric virus particles by SDS-PAGE analysis showed that 50 % of the coat proteins were fused to the HBsAg. Biological activity of the expressed HBsAg was assessed through the stimulation of in vitro antibody production by cultured peripheral blood mononuclear cells (PBMC). PBMC that were cultured in the presence of the chimeric virus showed up to an approximately three-fold increase in the level of anti HBsAg immunoglobulin thus suggesting the possible use of this new chimeric virus as an effective Hepatitis B vaccine.