Characterization of an excellent anti-Prelog short-chain dehydrogenase/reductase EbSDR8 from Empedobacter brevis ZJUY-1401
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- 作者：Aipeng Lia ; Lidan Yea ; b ; Hongping Wua ; Xiaohong Yanga ; Hongwei Yua ; yuhongwei@zju.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Empedobacter brevis ; Short-chain dehydrogenase/reductase ; Asymmetric reduction ; Anti-Prelog ; Cofactor regeneration
- 刊名：Journal of Molecular Catalysis B: Enzymatic
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：122
- 期：Complete
- 页码：179-187
- 全文大小：920 K
文摘
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A novel biocatalyst EbSDR8 with anti-Prelog stereoselectivity was reported.
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NADH can be efficiently regenerated via addition of isopropanol as cosubstrate.
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The recombinant whole-cell could catalyze the reaction without NADH addition.
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The biocatalyst exhibited excellent performance over a broad pH range of 7.0–10.5.
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EbSDR8 showed a broad substrate spectrum and excellent selectivity (>99% ee).