To explore genes specifically expressed in the bulge region, we classified a hair follicle into four areas, hair bulb, hair bulb to bulge (lower bulge), bulge, and epidermis to bulge (upper bulge), and collected these areas from back skin sections of C57BL/6 mice by laser microdissection. Real-time RT-PCR performed on these areas revealed that Frizzled (Fzd)-4, Fzd7, low density lipoprotein receptor-related protein 5 (Lrp5), and Lrp6, receptors for Wnt molecules, were expressed higher in the bulge area than other areas. Furthermore, FACS analysis showed that populations of Fzd4+ cells and Fzd7+ cells were different from those of Kit+ cells (precursor of melanocytes: melanoblasts). Fzd4+ and Fzd7+ cells isolated by FACS required a longer culture period to differentiate into mature melanocytes than Kit+ cells. Up-regulation of mRNA expressions of melanocyte markers (dopa chrometautomerase: Dct, tyrosinase: Tyr, tyrosinase-related protein 1: Tyrp1) was observed in Fzd4+ and Fzd7+ cells following Kit+ cells during differentiation. These results suggested that Fzd4+ and Fzd7+ cells were more immature than melanoblasts, therefore raising the possibility that Fzd4+ and Fzd7+ cells are MSCs.