Evaluating aqueous two-phase systems for Yarrowia lipolytica extracellular lipase purification

详细信息    查看全文

• 作者：Tamires Carvalho^a ; Priscilla V. Finotelli^b ; Renata C.F. Bonomo^c ; Marcelo Franco^d ; Priscilla F.F. Amaral^a ; ^{pamaral@eq.ufrj.br} ; ^{pffamaral@gmail.com}
• 关键词：AKEE ; acetone and kaolin enzyme extract ; ATPS ; aqueous two-phase system ; ATPS_EE ; aqueous two phase system enzyme extract ; CEE ; crude enzyme extract ; GRAS ; generally recognized as safe ; PEG ; polyethylene glycol ; p-NFL ; p-nitrophenyllaurate ; UEE ; ultrafiltrate enzyme extract ; YPD ; yeast extract ; peptone ; dextrose
• 刊名：Process Biochemistry
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：53
• 期：Complete
• 页码：259-266
• 全文大小：1155 K
• 卷排序：53

文摘

Lipase from Y. lipolytica was successfully purified with aqueous two-phase system. Lipase from Y. lipolytica was concentrated in the salty phase, easier to extract. Aqueous two-phase system (ATPS) method was superior to traditional methods. Lipase purified with ATPS showed good thermostability until 50 °C. Purified lipase extract showed good stability at a wide pH range.