The Arabidopsis nitrate transporter AtNRT2.1 is targeted to the root plasma membrane

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• 作者：Franck Chopin ; Judith Wirth ; Marie-France Dorbe ; Laurence Lejay ; Anne Krapp ; Alain Gojon ; Franç ; oise Daniel-Vedele
• 关键词：Nitrate transporter ; Arabidopsis thaliana ; GFP confocal imaging ; Antibodies
• 刊名：Plant Physiology and Biochemistry
• 出版年：2007
• 出版时间：August 2007
• 年：2007
• 卷：45
• 期：8
• 页码：630-635
• 全文大小：581 K

文摘

Arabidopsis AtNRT2.1 protein is the best characterized high affinity nitrate transporter in higher plants. However, nothing is known about its sub-cellular localization. In this work, we used GFP imaging to follow the targeting of the AtNRT2.1 protein to the different cell membranes. A polyclonal antibody was also raised against a peptide derived from the AtNRT2.1 sequence. Comparison of wild type and mutant plant extracts showed that this antibody recognized specifically the AtNRT2.1 protein. Microsomal membranes were fractionated on sucrose gradients and immunological detections were performed on the different fractions. Altogether, our results demonstrate that the AtNRT2.1 protein is located in the plasma membrane of the root cells.