Carboxyfullerene nanoparticles alleviate acute hepatic injury in severe hemorrhagic shock

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• 作者：Gan Chen ; Xiang Song ; Bo Wang ; Guoxing You ; Jingxiang Zhao ; Sha Xia ; Yuhua Zhang ; Lian Zhao ; ^{zhaolian@bmi.ac.cn" class="auth_mail" title="E-mail the corresponding author} ; Hong Zhou ; ^{zhouhtt1966@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Hemorrhagic shock ; Carboxyfullerene nanoparticles ; Acute hepatic injury ; Reactive oxygen species ; NF-κB pathway
• 刊名：Biomaterials
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：112
• 期：Complete
• 页码：72-81
• 全文大小：2032 K

文摘

Hemorrhagic shock/resuscitation involves overwhelming reactive oxygen species (ROS) that cause oxidative stress, inflammation, and subsequent tissue injury. We investigated the effects of the potent antioxidant carboxyfullerene (C₃) on acute liver injury during hemorrhage shock/resuscitation. C₃ infusion reduced the alanine aminotransferase (ALT) activity, methemoglobin content, malondialdehyde content, myeloperoxidase activity and expression levels of tumor necrosis factor -α and interleukin-6; it increased superoxide dismutase activity in the liver. The histologic injury score and apoptotic index were also markedly decreased after C₃ treatment compared with the vehicle group. Additionally, C₃-treated rats showed a significant decrease in nuclear factor-κB DNA binding capacity, which was preceded by reduced phosphorylation of the nuclear factor κB (NF-κB) p65 subunit in the liver. C₃ nanoparticles ameliorate oxidative stress, the inflammatory response, and subsequent acute liver injury after hemorrhagic shock/resuscitation. These protective effects appear to be mediated through the inhibition of the nuclear factor-κB pathway. C₃ treatment may be a promising strategy to improve tissue injury in hemorrhagic shock/resuscitation.