Metabolic fingerprinting of fresh lymphoma samples used to discriminate between follicular and diffuse large B-cell lymphomas

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• 作者：Ignasi Barba ; Carolina Sanz ; Angels Barbera ; Gustavo Tapia ; José ; -Luis Mate ; David Garcia-Dorado ; Josep-Maria Ribera ; Albert Oriol
• 刊名：Experimental Hematology
• 出版年：2009
• 出版时间：November 2009
• 年：2009
• 卷：37
• 期：11
• 页码：1259-1265
• 全文大小：742 K

文摘

Objective

To investigate if proton nuclear magnetic resonance (¹H NMR) spectroscopy−based metabolic profiling was able to differentiate follicular lymphoma (FL) from diffuse large B-cell lymphoma (DLBCL) and to study which metabolites were responsible for the differences.

Materials and Methods

High-resolution ¹H NMR spectra was obtained from fresh samples of lymph node biopsies obtained consecutively at one center (14 FL and 17 DLBCL). Spectra were processed using pattern-recognition methods.

Results

Discriminant models were able to differentiate between the two tumor types with a 86 % sensitivity and a 76 % specificity; the metabolites that most contributed to the discrimination were a relative increase of alanine in the case of DLBCL and a relative increase of taurine in FL. Metabolic models had a significant but weak correlation with Ki67 expression (r² = 0.42; p = 0.002)

Conclusions

We have proved that it is possible to differentiate between FL and DLBCL based on their NMR metabolic profiles. This approach may potentially be applicable as a noninvasive tool for diagnostic and treatment follow-up in the clinical setting using conventional magnetic resonance systems.