The Clostridium botulinum GerAB germination protein is located in the inner membrane of spores

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• 作者：Franç ; ois Alberto ; Lucien Botella ; Fré ; deric Carlin ; Christophe Nguyen-the and Vé ; ronique Broussolle
• 关键词：Clostridium botulinum ; Spore ; Germination ; GerAB ; Membrane protein
• 刊名：FEMS Microbiology Letters
• 出版年：2005
• 出版时间：15 December 2005
• 年：2005
• 卷：253
• 期：2
• 页码：231-235
• 全文大小：191 K

文摘

Clostridium botulinum dormant spores germinate in presence of l-alanine via a specific receptor composed of GerAA, GerAB and GerAC proteins. In Bacillus subtilis spores, GerAA and GerAC proteins were located in the inner membrane of the spore. We studied the location of the GerAB protein in C. botulinum spore fractions by Western-blot analysis, using an antipeptidic antibody. The protein GerAB was in vitro translated and used to confirm the specificity of the antibodies. GerAB was not present in a coat and spore outer membrane fraction but was present in a fraction of decoated spores containing inner membrane. These results strongly suggest that the protein GerAB is located in the inner membrane of the spore.