Stable reference genes are necessary to a reliable expression data by quantitative PCR.
We determined a set of reference genes for qPCR use in injured and non-injured menisci.
NormFinder, geNorm, BestKeeper DataAssist and RefFinder software packages and comparative ΔCt. method were used.
HPRT1 + TBP + GAPDH is the most suitable combination for the analysis of injured and non-injured menisci.