Enzyme inhibition studies by integrated Michaelis-Menten equation considering simultaneous presence of two inhibitors when one of them is a reaction product
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- 作者：Rui M.F. Bezerra ; bezerra@utad.pt" class="auth_mail" title="E-mail the corresponding author ; Paula A. Pinto ; Irene Fraga ; Albino A. Dias
- 关键词：Integrated Michaelis&ndash ; Menten equations ; Linear inhibition ; General mechanism kinetics ; Diagnosis of enzyme inhibition ; Two inhibitors
- 刊名：Computer Methods and Programs in Biomedicine
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：125
- 期：Complete
- 页码：2-7
- 全文大小：606 K
文摘
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A method for the study of enzyme kinetics in the presence of two inhibitors.
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Integrated Michaelis–Menten equations were developed to carry out the analysis.
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This methodology is useful to discriminate the inhibition mechanisms.
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This method can be automated and applied in real time.
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An Excel template has been developed (available in Appendix).