Low-molecular-weight fractions of Alcalase hydrolyzed egg ovomucin extract exert anti-inflammatory activity in human dermal fibroblasts through the inhibition of tumor necrosis factor-mediated nuclear factor κB pathway

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• 作者：Xiaohong Sun^a ; ¹ ; Subhadeep Chakrabarti^a ; ^b ; ¹ ; Jun Fang^c ; ^d ; Yulong Yin^c ; ^d ; Jianping Wu^a ; ^b ; ^{jwu3@ualberta.ca" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Ovomucin hydrolysate ; Inflammation ; Dermal fibroblast ; Desalting ; NF-κB ; Ultrafiltration
• 刊名：Nutrition Research
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：36
• 期：7
• 页码：648-657
• 全文大小：783 K

文摘

Ovomucin is a mucin-like protein from egg white with a variety of biological functions. We hypothesized that ovomucin-derived peptides might exert anti-inflammatory activity. The specific objectives were to test the anti-inflammatory activities of different ovomucin hydrolysates and its various fractions in human dermal fibroblasts, and to understand the possible molecular mechanisms. Three ovomucin hydrolysates were prepared and desalted; only the desalted Alcalase hydrolysate showed anti-inflammatory activity. Desalting of ovomucin hydrolysate enriched the proportion of low-molecular-weight (MW) peptides. Indeed, ultrafiltration of this hydrolysate displayed comparable anti-inflammatory activity in dermal fibroblasts, indicating the responsible role of low-MW bioactive peptides in exerting the beneficial biological function. The anti-inflammatory activity of low-MW peptides was regulated through the inhibition of tumor necrosis factor–mediated nuclear factor κ–light-chain-enhancer of activated B cells activity. Our study demonstrated that both peptide composition and MW distribution play important roles in anti-inflammatory activity. The low-MW fractions prepared from ovomucin Alcalase hydrolysate may have potential applications for maintenance of dermal health and treatment of skin diseases.