Genome-wide analysis reveals increased levels of transcripts related with infectivity in peanut lectin non-agglutinated promastigotes of Leishmania infantum

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• 作者：Pedro J. Alcolea ; Ana Alonso ; Alicia Sá ; nchez-Gorostiaga ; Mercedes Moreno-Paz ; Manuel J. Gó ; mez ; Irene Ramos ; Ví ; ctor Parro ; Vicente Larraga
• 关键词：Leishmania infantum ; Shotgun DNA microarrays ; Peanut lectin agglutination ; Metacyclic promastigotes ; Infectivity
• 刊名：Genomics
• 出版年：2009
• 出版时间：June 2009
• 年：2009
• 卷：93
• 期：6
• 页码：551-564
• 全文大小：1262 K

文摘

Metacyclic promastigotes are transmitted during bloodmeals after development inside the gut of the sandfly vector. The isolation from axenic cultures of procyclic and metacyclic promastigotes by peanut lectin agglutination followed by differential centrifugation is controversial in Leishmania infantum. The purpose of this study has been to isolate both fractions simultaneously from the same population in stationary phase of axenic culture and compare their expression profiles by whole-genome shotgun DNA microarrays. The 317 genes found with meaningful values of stage-specific regulation demonstrate that negative selection of metacyclic promastigotes by PNA agglutination is feasible in L. infantum and both fractions can be isolated. This subpopulation up-regulates a cysteine peptidase A and several genes involved in lipophosphoglycan, proteophosphoglycan and glycoprotein biosynthesis, all related with infectivity. In fact, we have confirmed the increased infection rate of PNA⁻ promastigotes by U937 human cell line infection experiments. These data support that metacyclic promastigotes are related with infectivity and the lack of agglutination with PNA is a phenotypic marker for this subpopulation.