Functional Analysis via Standardized Whole-Blood Stimulation Systems Defines the Boundaries of a Healthy Immune Response to Complex Stimuli

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• 作者：Darragh Duffy¹ ; ² ; ³ ; ¹⁴ ; Vincent Rouilly¹ ; ⁴ ; ¹⁴ ; Valentina Libri¹ ; ¹⁴ ; Milena Hasan¹ ; Benoit Beitz¹ ; Mikael David¹ ; Alejandra Urrutia¹ ; ² ; ³ ; Auré ; lie Bisiaux² ; ³ ; Samuel T. LaBrie⁵ ; Annick Dubois⁶ ; Ivo G. Boneca⁷ ; ⁸ ; Cé ; cile Delval⁶ ; Sté ; phanie Thomas¹ ; ² ; ³ ; Lars Rogge¹ ; ⁹ ; Manfred Schmolz¹⁰ ; Lluis Quintana-Murci¹¹ ; ¹² ; ¹⁵ ; ^{quintana@pasteur.fr" class="auth_mail} ; Matthew L. Albert¹ ; ² ; ³ ; ¹³ ; ¹⁵ ; ^{albertm@pasteur.fr" class="auth_mail} ; for The Milieu Inté ; rieur Consortium Laurent Abel ; Andres Alcover ; Philippe Bousso ; Ana Cumano ; Marc Daë ; ron ; Cé ; cile Delval ; Caroline Demangel ; Ludovic Deriano ; James Di Santo ; Franç ; oise Dromer ; Gé ; rard Eberl ; Jost Enninga ; Antonio Freitas ; Ivo Gomperts-Boneca ; Serge Hercberg ; Olivier Lantz ; Claude Leclerc ; Hugo Mouquet ; Sandra Pellegrini ; Stanislas Pol ; Lars Rogge ; Anavaj Sakuntabhai ; Olivier Schwartz ; Benno Schwikowski ; Spencer Shorte ; Vassili Soumelis ; Fré ; dé ; ric Tangy ; Eric Tartour ; Antoine Toubert ; Marie-Noë ; lle Ungeheuer ; Lluis Quintana-Murci ; Matthew L. Albert
• 刊名：Immunity
• 出版年：20 March, 2014
• 年：2014
• 卷：40
• 期：3
• 页码：436-450
• 全文大小：3589 K

文摘

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Summary

Standardization of immunophenotyping procedures has become a high priority. We have developed a suite of whole-blood, syringe-based assay systems that can be used to reproducibly assess induced innate or adaptive immune responses. By eliminating preanalytical errors associated with immune monitoring, we have defined the protein signatures induced by (1) medically relevant bacteria, fungi, and viruses; (2) agonists specific for defined host sensors; (3) clinically employed cytokines; and (4) activators of T聽cell immunity. Our results provide an initial assessment of healthy donor reference values for induced cytokines and chemokines and we report the failure to release interleukin-1伪 as a common immunological phenotype. The observed naturally occurring variation of the immune response may help to explain differential susceptibility to disease or response to therapeutic intervention. The implementation of a general solution for assessment of functional immune responses will help support harmonization of clinical studies and data sharing.