Genetic polymorphism of matrix metalloproteinase (MMP)-9 does not affect plasma MMP-9 activity in healthy subjects

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• 作者：Caroline Demacq ; Ana P. de Souza ; Alcyone A. Machado ; Raquel F. Gerlach and Jose E. Tanus-Santos
• 刊名：Clinica Chimica Acta
• 出版年：2006
• 出版时间：March 2006
• 年：2006
• 卷：365
• 期：1-2
• 页码：183-187
• 全文大小：124 K

文摘

Background

Plasma MMP-9 levels have been shown to predict cardiovascular risk, and a functional substitution C to T at position − 1562 in the promoter region of the MMP-9 gene has been associated with the severity of cardiovascular diseases. We examined the association between the C^− 1562T polymorphism and MMP-9 activity in healthy subjects.

Methods

We studied 200 healthy male white volunteers (age range: 20–55 y) who were nonsmokers and were not taking medicines. Genomic DNA was extracted and genotypes for the C^− 1562T polymorphism were determined by PCR and restriction fragment length digestion. Plasma was assayed for pro-MMP-9 and MMP-9 activities by gelatin zymography.

Results

The frequency of the alleles “C” and “T” were 90 % and 10 % , respectively. Because of the relatively low frequency of the TT genotype, we combined both TT and CT genotypes together (CT + TT group) and compared with the CC genotype group. We found no differences in pro-MM9 and MMP-9 activity levels among the genotype groups (both P > 0.05).

Conclusions

While the present study indicates lack of effect for the C^− 1562T polymorphism on MMP-9 activity in plasma, it is possible that the C^− 1562T polymorphism contributes to an increased cardiovascular risk under conditions of induced MMP-9 expression.