Protein engineering of aldolase LbDERA for enhanced activity toward real substrates with a high-throughput screening method coupled with an aldehyde dehydrogenase
刊名:Biochemical and Biophysical Research Communications
出版年:2017
出版时间:1 January 2017
年:2017
卷:482
期:1
页码:159-163
全文大小:828 K
卷排序:482
文摘
A high-throughput method for screening aldolase variants was established. A variant LbDERAT29L/F163Y with 7-fold improved activity was identified. The crystal structure reveals structure changes responsible for activity improvement.