AntiHepatitis C Virus Activity of Alectryon serratus Leaves Extract

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• 作者：Lidya Tumewu^a ; Evhy Apryani^a ; Mei Ria Santi^c ; Tutik Sri Wahyuni^a ; ^b ; Adita Ayu Permanasari^a ; Myrna Adianti^a ; Chie Aoki^d ; Aty Widyawaruyanti^a ; ^b ; Achmad Fuad Hafid^a ; ^b ; ^{achmadfuad@yahoo.com" class="auth_mail" title="E-mail the corresponding author} ; Maria Inge Lusida^a ; Soetjipto^a ; Hak Hotta^d
• 关键词：Alectryon serratus ; leaves extract ; antiHepatitis C Virus
• 刊名：Procedia Chemistry
• 出版年：2016
• 出版时间：2016
• 年：2016
• 卷：18
• 期：Complete
• 页码：169-173
• 全文大小：144 K

文摘

Hepatitis C Virus (HCV) has infected approximately 2-3% (130-170 million) of the world's population. No vaccine is available to prevent HCV infection. Investigation of anti-HCV agent is thus deemed necessary. Various plants have been explored for their anti-HCV activity. A. serratus is a member of Sapindaceae family, which fruit and seed were traditionally used as insecticide. Anti-HCV activity tested on A.serratus leaves extract has been done. The result showed that leaves extract exhibited anti-HCV with IC₅₀ value of 14.9 μg/ml and 9.8 μg/ml against HCV J6/JFH1 and JFH1a, respectively. The cytotoxicity assay results showed that A.serratus leaves extract was not toxic and has CC₅₀ >100 μg/ml. Mode of action experiment results suggested that A.serratus extract inhibited HCV at the post-entry step. Further fractionation of leaves extract by open column chromatography resulted in 4 fractions. Only Fraction 1 (AP-5F.1) exhibited anti-HCV with IC₅₀ value of 1.2 μg/ml against HCV JFH1a. Separation of AP-5F.1 by open column chromatography resulted in 15 fractions. Fraction number 13 (AP-5F.1.13) exhibited anti-HCV with IC₅₀ value of 0.43 μg/ml against HCV JFH1a. Separation of AP-5F.1.13 by semi preparative-HPLC resulted in isolate identified by TLC and LC-MS method as chlorophyll derivate. There was a possibility that chlorophyll derivate has participated in performing the anti-HCV activity of fractions and extract besides the other compounds contained. In this study, we concluded that A. serratus leaves extract, AP-5F.1, and AP-5F.1.13 exhibited anti-HCV activity against JFH1a virus.