Peculiarities of the interaction of the restriction endonuclease BspD6I with DNA containing its recognition site
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- 作者：Liudmila A. Abrosimovaa ; abrludmila@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Elena A. Kubarevaa ; kubareva@belozersky.msu.ru" class="auth_mail" title="E-mail the corresponding author ; Anzhela Yu. Migura ; anzhelamigur@mail.ru" class="auth_mail" title="E-mail the corresponding author ; Aleksandra V. Gavshinaa ; saschagawschina@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Aleksandra Yu. Ryazanovaa ; venel.ale@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Maxim V. Norkina ; b ; norkin_1991@mail.ru" class="auth_mail" title="E-mail the corresponding author ; Tatiana A. Perevyazovac ; anatolia19@mail.ru" class="auth_mail" title="E-mail the corresponding author ; Wolfgang Wended ; wolfgang.wende@chemie.bio.uni-giessen.de" class="auth_mail" title="E-mail the corresponding author ; Tibor Hianike ; tibor.hianik@fmph.uniba.sk" class="auth_mail" title="E-mail the corresponding author ; Liudmila A. Zheleznayac ; la_zheleznaya@mail.ru" class="auth_mail" title="E-mail the corresponding author ; Tatiana S. Oretskayaa ; oretskaya@belozersky.msu.ru" class="auth_mail" title="E-mail the corresponding author
- 关键词：AFM ; atomic-force microscopy ; Bt ; biotin residue ; EDTA ; ethylenediaminetetraacetic acid ; EMSA ; electrophoretic mobility shift assay ; IC50 ; half-maximal inhibitory concentration ; Kd(app) ; apparent dissociation constant ; MTase ; M. ; methyltransferase ; m6A ; 6-methyl-2&prime ; -deoxyadenosine ; NE ; Nt. ; nicking endonuclease ; PAG ; polyacrylamide gel ; RE ; R. ; restriction endonuclease ; R-M ; restriction-modification ; SD ; standard deviation ; SDS ; sodium dodecyl sulfate ; SE ; standard error of the mean ; ss. ; s
- 刊名：Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：1864
- 期：9
- 页码：1072-1082
- 全文大小：1506 K
文摘
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BspD6I enzyme efficiently cleaves DNA recognition site flanking by 2 nucleotide pairs.
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BspD6I nicking endonuclease bends DNA in the process of complex formation.
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BspD6I small subunit is able to hydrolyze a substrate after its nicking.
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Two subunits are the reliable partners in effective DNA cleavage by BspD6I enzyme.