Structural Insights into the Inhibitory Mechanism of an Antibody against B7-H6, a Stress-Induced Cellular Ligand for the Natural Killer Cell Receptor NKp30
详细信息 查看全文
- 作者：Xiaoping Xu1 ; 2 ; &dagger ; ; Emilie Narni-Mancinelli3 ; &dagger ; ; Claudia Cantoni4 ; 5 ; 6 ; &dagger ; ; Yili Li1 ; 7 ; &dagger ; ; Sophie Guia3 ; Laurent Gauthier8 ; Qianming Chen2 ; Alessandro Moretta4 ; 5 ; Fré ; dé ; ric Vé ; ly3 ; 9 ; Edward Eisenstein1 ; 10 ; Sneha Rangarajan1 ; 7 ; Eric Vivier3 ; vivier@ciml.univ-mrs.fr" class="auth_mail" title="E-mail the corresponding author ; Roy A. Mariuzza1 ; 7 ; rmariuzz@umd.edu" class="auth_mail" title="E-mail the corresponding author ; mariuzza@ibbr.umd.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：NK ; natural killer ; MHC ; major histocompatibility complex ; MHC-I ; major histocompatibility complex class I ; IgV ; Ig variable ; CDR ; complementarity-determining region ; IgG ; Immunoglobulin G ; mAb ; monoclonal antibody ; V ; variable ; C ; constant ; Fab ; antigen-binding fragment ; wt ; wild-type ; FcR ; Fc receptor ; ORF ; open reading frame
- 刊名：Journal of Molecular Biology
- 出版年：2016
- 出版时间：6 November 2016
- 年：2016
- 卷：428
- 期：22
- 页码：4457-4466
- 全文大小：1299 K
文摘
- •
NK cell receptor NKp30 recognizes stress-induced ligand B7-H6 on tumor cells.
- •
Monoclonal anti-B7-H6 antibody blocks NKp30-mediated NK cell activation.
- •
We determined the structure of antibody–B7-H6 complex to elucidate inhibitory mechanism.
- •
Antibody binding site on B7-H6 is completely distinct from NKp30-binding site.
- •
Antibody interferes with cell–cell contacts required for NK cell activation.