The prevalence of cerebral coenurosis was 39.7 % (27/68). Migratory lesions were found in 23 sheep (85.1 % ), cystic lesions in 3 sheep (11.2 % ) and both lesions were found in 1 sheep (3.7 % ). The most abundant isoenzymes detected both in controls and in sheep with cerebral coenurosis were CK-MM, Macro-CK2 and CK-BB. No significant differences regarding absolute values of CK-BB were found suggesting that CK-BB is not released in serum during cerebral coenurosis. Conversely, CK-MM was significantly lower (P < 0.05) in sheep with cerebral coenurosis compared with controls, inducing a relative increase of the percentage of CK-BB (P < 0.05). This latter increase had a good discriminating power (area under the ROC curve: 0.71; P < 0.000). Although at its best diagnostic performance (CK-BB higher than 24,4 % ) sensitivity and specificity were moderate (66.7 % and 68.3 % , respectively), the likelihood ratio of increased CK-BB was 2.10. The increased percentage of CK-BB can thus be useful in the diagnostic approach to suspected cases of cerebral coenurosis, when the pre-test probability of disease is high.