Contrasting microsatellite diversity in the evolutionary lineages of Phytophthora lateralis

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• 作者：AnnaMaria Vettraino^a ; Clive M. Brasier^b ; Joan F. Webber^b ; Everett M. Hansen^c ; Sarah Green^d ; Cecile Robin^e ; ^f ; Alessia Tomassini^a ; Natalia Bruni^a ; Andrea Vannini^a ; ^{vannini@unitus.it}
• 关键词：Genetic variation ; Hybridization ; Introduction ; Evolution ; Microsatellite analysis
• 刊名：Fungal Biology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：121
• 期：2
• 页码：112-126
• 全文大小：623 K
• 卷排序：121

文摘

Following recent discovery of Phytophthora lateralis on native Chamaecyparis obtusa in Taiwan, four phenotypically distinct lineages were discriminated: the Taiwan J (TWJ) and Taiwan K (TWK) in Taiwan, the Pacific Northwest (PNW) in North America and Europe and the UK in west Scotland. Across the four lineages, we analysed 88 isolates from multiple sites for microsatellite diversity. Twenty-one multilocus genotypes (MLGs) were resolved with high levels of diversity of the TWK and PNW lineages. No alleles were shared between the PNW and the Taiwanese lineages. TWK was heterozygous at three loci, whereas TWJ isolates were homozygous apart from one isolate, which exhibited a unique allele also present in the TWK lineage. PNW lineage was heterozygous at three loci. The evidence suggests its origin may be a yet unknown Asian source. North American and European PNW isolates shared all their alleles and also a dominant MLG, consistent with a previous proposal that this lineage is a recent introduction into Europe from North America. The UK lineage was monomorphic and homozygous at all loci. It shared its alleles with the PNW and the TWJ and TWK lineages, hence a possible origin in a recent hybridisation event between a Taiwan lineage and PNW cannot be ruled out.