Inhibition of multispecies biofilms by a fluoride-releasing dental prosthesis copolymer

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• 作者：Sufian A. Yassin^a ; ^{drsufian2005@yahoo.com" class="auth_mail" title="E-mail the corresponding author} ; Matthew J. German^a ; ^{matthew.german@newcastle.ac.uk" class="auth_mail" title="E-mail the corresponding author} ; Sarah L. Rolland^a ; ^{s.l.rolland@newcastle.ac.uk" class="auth_mail" title="E-mail the corresponding author} ; Alexander H. Rickard^b ; ^{alexhr@umich.edu" class="auth_mail" title="E-mail the corresponding author} ; Nicholas S. Jakubovics^a ; ^{nick.jakubovics@newcastle.ac.uk" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Antibacterial agents ; Biofilms ; Confocal laser scanning microscopy ; Dental plaque ; Fluoride ; Microbiology
• 刊名：Journal of Dentistry
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：48
• 期：Complete
• 页码：62-70
• 全文大小：1719 K

文摘

This study aimed to develop a new mixed-species acidogenic biofilm model and use it to assess the antimicrobial properties of a novel fluoride-releasing copolymer.

Methods

Stubs composed of a copolymer of methyl methacrylate (MMA) and 2-hydroxyethyl methacrylate (HEMA) with polymethyl methacrylate (PMMA) were produced by chemically-activated free radical polymerization. A fluoride-releasing copolymer was developed by incorporating sodium fluoride in place of a portion of the PMMA. Samples were mounted in polysulfone Modified Robbins Devices (MRDs) and were optimized for single- and mixed-species biofilm formation by Candida albicans, Lactobacillus casei and Streptococcus mutans.

Results

Fluoride release was sustained for at least 48 h in flowing conditions. Fluoride did not affect the colonization and biofilm growth of any of the microorganisms in monocultures. However, in mixed-species biofilms, cell densities of all three species were reduced approximately ten-fold (p < 0.05) on the fluoridated material compared with the non-fluoridated copolymer.

Conclusions

These data demonstrate that intermicrobial interactions in mixed-species acidogenic biofilms are sensitive to fluoride, and that the inclusion of fluoride in a denture lining copolymer reduces the formation of polymicrobial biofilms.

Clinical significance

The growth of acidogenic microorganisms on denture materials is associated with denture stomatitis and dental caries on surrounding teeth. A fluoride-releasing copolymer that inhibits acidogenic mixed-species biofilms, such as the material described in this study, has the potential to control these diseases by limiting biofilm growth.