Differential detection of pathogenic Yersinia spp. by fluorescence in situ hybridization

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• 作者：Alexander Rohde^a ; ^b ; ^{alexander.rohde@bfr.bund.de} ; Jens Andre Hammerl^a ; Bernd Appel^a ; Ralf Dieckmann^a ; Sascha Al Dahouk^a
• 关键词：Yersinia ; FISH ; Food safety ; Direct viable count
• 刊名：Food Microbiology
• 出版年：2017
• 出版时间：April 2017
• 年：2017
• 卷：62
• 期：Complete
• 页码：39-45
• 全文大小：938 K
• 卷排序：62

文摘

Yersinia detection in food by cultural methods is tedious and time-consuming. FISH is an efficient alternative method to detect pathogenic Yersinia. Thermodynamic modelling allows for multiplex FISH assays. Competitors, LNAs and dual stainings make FISH more reliable. DVC-FISH enables superior live/dead differentiation.