Prohormone convertase-1 is essential for conversion of chromogranin A to pancreastatin
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- 作者：Udupi ; Vidyavathi ; Lee ; Heung-Man ; Kurosky ; Alexander ; Greeley Jr ; George H.
- 关键词：Peptide ; Processing ; Endoprotease ; Antisense
- 刊名：Regulatory Peptides
- 出版年：1999
- 出版时间：September 15, 1999
- 年：1999
- 卷：83
- 期：2-3
- 页码：123-127
- 全文大小：131 K
文摘
The purpose of this study was to test the hypothesis that the endoprotease, prohormone convertase-1 (PC-1), is involved in the processing of the precursor protein chromogranin A (CGA) to a smaller peptide called pancreastatin (PST). A human pancreatic carcinoid cell line (BON) that expresses PC-1, CGA and PST was stably transfected with antisense PC-1 mRNA. BON cells expressing antisense PC-1 mRNA showed nearly complete abolishment of PC-1 protein (
95 % reduction) and an 80 % reduction in cell content of PST immunoreactivity (PST-IR) as assessed by high-performance liquid chromatography in combination with measurement of PST-IR. These findings indicate that PC-1 is essential for processing CGA to PST.
95 % reduction) and an 80 % reduction in cell content of PST immunoreactivity (PST-IR) as assessed by high-performance liquid chromatography in combination with measurement of PST-IR. These findings indicate that PC-1 is essential for processing CGA to PST.