Identification of a 24 kDa intrinsic membrane protein from mammalian peroxisomes
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- 作者：Reguenga ; Carlos ; Oliveira ; Má ; rcia E.M. ; Gouveia ; Alexandra M.M. ; Eckerskorn ; Christoph ; et. al.
- 关键词：Peroxisome ; Peroxisomal membrane protein ; (Mammal) ; (Rat liver) ; mPTS ; peroxisomal targeting signal of membrane proteins ; PMPX ; peroxisomal membrane protein of X kDa ; Pex ; peroxin ; EST ; expressed sequence tag ; STS ; sequence tagged site
- 刊名：Biochimica et Biophysica Acta (BBA)/Gene Structure and Expression
- 出版年：1999
- 出版时间：June 9, 1999
- 年：1999
- 卷：1445
- 期：3
- 页码：337-341
- 全文大小：430 K
文摘
A 24 kDa protein from rat liver peroxisomal membrane was isolated and subjected to Edman degradation. Using the N-terminal sequence of this polypeptide we have identified several rat and human expressed sequence tags in the GenBank Database. The complete sequence of a human cDNA clone was determined. The open reading frame encodes an extremely basic protein 212 amino acid residues long. A high similarity between this mammalian protein and hypothetical proteins from Caenorhabditis elegans and Neurospora crassa was found. Hydropathy analysis reveals the existence of two putative membrane-spanning domains in conserved regions of the three homologous proteins.