Effect of acute phase response induction by turpentine oil administration on hepatocyte regeneration after partial hepatectomy in the rat

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• 作者：Theocharis ; Stamatios E. ; Margeli ; Alexandra P. ; Skaltsas ; Spyridon D. ; Kittas ; Christos N.
• 关键词：Acute-phase response ; Partial heptectomy ; DNA ; TK ; PCNA
• 刊名：International Hepatology Communications
• 出版年：1997
• 出版时间：July, 1997
• 年：1997
• 卷：7
• 期：3
• 页码：188-196
• 全文大小：572 K

文摘

The aim of the present study was to examine whether acute-phase response induction by turpentine oil administration, affects hepatocyte regeneration after partial hepatectomy (PH) in rats. It is known that administration of turpentine oil in rats induces acute-phase response, with maximum circulation of acute-phase reactants 24 h after the injection. Turpentine oil was injected in rats subcutaneously, either 24 h prior to or simultaneous with 70 % PH. The regenerative capacity of hepatocytes was estimated 24 h postoperatively, by the in-vivo incorporation of tritiated thymidine into liver DNA, the enzymatic activity of liver thymidine kinase (TK), the mitotic index and the percentage of positive for proliferating cell nuclear antigen (PCNA) nuclei of hepatocytes. When turpentine oil was administered in rats 24 h prior to PH, an increase of hepatocyte proliferation, evident from tritiated thymidine incorporation into liver DNA and TK activity was observed, P < 0.001, compared with that found in simply partially hepatectomized ones. The administration of turpentine oil simultaneous with PH did not further affect the proliferation of hepatocytes. This fact suggests that a stimulatory effect of acute phase response induction on hepatocyte proliferation occurs, when the maximum circulation of acute-phase reactants coincides with the time point of PH, enhancing by this way the hepatocyte replicative capacity.