Action of anti-bothropic factor isolated from Didelphis marsupialis on renal effects of Bothrops erythromelas venom

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• 作者：Alice M.C. Martins ; Fabiola C.M. Sousa ; Paulo S.F. Barbosa ; Marcos H. Toyama ; Daniela O. Toyama ; Cleidiana C. Aprí ; gio ; Maria G.R. Queiroz ; Mirian C. Guarnieri ; Alexandre Havt ; Dalgimar B. de Mene
• 关键词：Anti-bothropic factor ; Bothrops erytromrlas ; Renal effects
• 刊名：Toxicon
• 出版年：2005
• 出版时间：2005
• 年：2005
• 卷：46
• 期：6
• 页码：595-599
• 全文大小：96 K

文摘

Acute renal failure is the most common complication in the lethal cases caused by snakebites in Brazil. Among the Brazilian venom snakes, Bothrops erythromelas is responsible for the majority of accidents in Northeastern Brazil. Didelphis marsupialis serum could inhibit myonecrotic, hemorrhagic, edematogenic hyperalgesic and lethal effects of envenomation determined by ophidian bites. In the present study, we evaluated the action of the anti-bothropic factor isolated from D. marsupialis on the renal effects promoted by B. erythromelas venom without systemic interference. Isolated kidneys from Wistar rats were perfused with Krebs–Henseleit solution containing 6 % bovine serum albumin. We analyzed renal perfusion pressure (PP), renal vascular resistance (RVR), glomerular filtration rate (GFR), urinary flow (UF), and the percentages of sodium and potassium tubular transport ( % TNa⁺, % TK⁺). The B. erythromelas venom (10 μg mL⁻¹) decreased the PP (ct=108.71±5.09 mmHg; BE=65.21±5.6 mmHg*) and RVR (ct=5.76±0.65 mmHg mL⁻¹ g⁻¹ min⁻¹; BE=3.10±0.45 mmHg mL⁻¹ g⁻¹ min⁻¹*). On the other hand, the GFR decreased at 60 min (ct₆₀