Monitoring a toxic bloom of Alexandrium minutum using novel microarray and multiplex surface plasmon resonance biosensor technology
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- 作者：Gary R. McCoy ; Sara McNamee ; Katrina Campbell ; Christopher T. Elliott ; Gerard T.A. Fleming ; Robin Raine
- 关键词：PSP ; paralytic shellfish poisoning ; FISH ; Fluorescent in situ Hybridisation ; qRT-PCR ; quantitative real-time polymerase chain reaction ; RNA ; ribonucleic acid ; DNA ; deoxyribonucleic acid ; MIDTAL ; Microarrays for the Detection of Toxic Algae ; multi SPR ; multiplex surface plasmon resonance ; ELISA ; enzyme-linked immunosorbent assay ; HPLC ; high performance liquid chromatography ; Poly (dT) tail ; poly (deoxythymidylic) tail ; BCP ; 1-bromo-3-chloro-propane ; S/N ratio ; signal-to-noise ratio ; STX ; saxitoxi
- 刊名：Harmful Algae
- 出版年：February, 2014
- 年：2014
- 卷：32
- 期：Complete
- 页码：40-48
- 全文大小：1293 K
文摘
Blooms of Alexandrium occur annually during the summer months in the North Channel of Cork Harbour on the south coast of Ireland. This study monitored an extensive bloom of the toxin producing Alexandrium minutum during the summer of 2011 with the use of the MIDTAL (Microarrays for the Detection of Toxic Algae) microarray and a prototype multiplex surface plasmon resonance (multi SPR) biosensor. Microarray signal intensities and toxin results from three testing platforms of the prototype multi SPR biosensor, commercial (CER) enzyme-linked immunosorbent assay (ELISA) and high performance liquid chromatography (HPLC) were compared against light microscopy counts. The main aim was to demonstrate the use of these methodologies to support national monitoring agencies by providing a faster and more accurate means of identifying and quantifying the harmful phytoplankton community and their toxins in natural water samples. Both the microarray signals and multi SPR biosensor results followed a significant trend with light microscopy results and both techniques indicated detection limits of <4000 cells of A. minutum in natural seawater samples.