Establishing the subcellular localization of photodynamically-induced ROS using 3,3′-diaminobenzidine: A methodological proposal, with a proof-of-concept demonstration
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- 作者：Juan C. Stockerta ; b ; juancarlos.stockert@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Alfonso Blá ; zquez-Castroc
- 关键词：ROS ; 3 ; 3&prime ; -Diaminobenzidine ; Colorimetric reaction ; Localized ROS generation ; Photosensitizers ; Photoactive dyes
- 刊名：Methods
- 出版年：2016
- 出版时间：15 October 2016
- 年：2016
- 卷：109
- 期：Complete
- 页码：175-179
- 全文大小：1115 K
文摘
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Current methods for detecting cellular ROS production are not site-specific.
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Oxidation of 3,3′-diaminobenzidine (DAB) results in a localized signal.
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A proof-of-concept method for site-specific subcellular ROS detection is reported.
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The method is based on DAB oxidation by a photodynamic compound.
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A general proposal for site-specific ROS detection based on this method is proposed.