Liquid-liquid phase separation of a monoclonal antibody at low ionic strength: Influence of anion charge and concentration

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• 作者：Katharina Reiche^a ; ^b ; Josef Hartl^a ; Alfred Blume^b ; Patrick Garidel^a ; ^b ; ^{patrick.garidel@boehringer-ingelheim.com}
• 关键词：Liquid-liquid phase separation ; Monoclonal antibody ; Oligovalent anion ; Citrate ; Upper critical solution temperature (UCST) ; Critical opalescence
• 刊名：Biophysical Chemistry
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：220
• 期：Complete
• 页码：7-19
• 全文大小：1015 K
• 卷排序：220

文摘

Antibody solution undergoes liquid-liquid phase separation (LLPS) at the isoelectric point. Citrate and oligovalent anions induce LLPS at pH below the isoelectric point. Citrate is assumed to bind to the antibody resulting in charge neutralization. Citrate-antibody binding was complementarily proven by using buffer equilibration.