Development of molecular tools to monitor conjugative transfer in rhizobia
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- 作者：Gonzalo Torres Tejerizoa ; gatt@biol.unlp.edu.ar" class="auth_mail" title="E-mail the corresponding author ; Luis Alfredo Bañ ; uelosb ; Laura Cervantesb ; Paul Gaytá ; nc ; Mariano Pistorioa ; David Romerob ; Susana Bromb
- 关键词：Rhizobia ; Plasmid ; Conjugative transfer ; Fluorescent cassettes
- 刊名：Journal of Microbiological Methods
- 出版年：2015
- 出版时间：October 2015
- 年：2015
- 卷：117
- 期：Complete
- 页码：155-163
- 全文大小：1531 K
文摘
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24 cassettes with promoter–fluorescent protein–antibiotic were developed.
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Conditions to separate transconjugants by flow cytometry (FC) were established.
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Conjugative frequency values obtained by FC and viable counts were similar.
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Microscopic observations allowed the detection of transconjugants on bean roots.