Parathyroid hormone-like hormone (PTHLH) exerts relevant roles in pro
gression and dissemination of several tumors. However, factors influencin
g its production and secretion have not been fully characterized. The main limitation is the lack of specific, sensitive and widely available techniques to detect and quantify PTHLH. We have developed a lateral flow immunoassay usin
g gold nanoparticles label for the fast and easy detection of PTHLH in lysates and culture media of three human cell lines (HaCaT, LA-N-1, SK-N-AS). Levels in culture media and lysates ran
ged from 11 to 20 n
g/mL and 0.66 to 0.87 μ
g/mL respectively. Results for HaCaT are in a
greement to the previously reported, whereas LA-N-1 and SK-N-AS have been evaluated for the first time. The system also exhibits
good performance in human serum samples. This methodolo
gy represents a helpful tool for future
in vitro and
in vivo studies of mechanisms involved in PTHLH production as well as for dia
gnostics.
From the Clinical Editor
Parathyroid Hormone-like Hormone (PTHLH) is known to be secreted by some tumors. However, the detection of this peptide remains difficult. The authors here described their technique of using gold nanoparticles as label for the detection of PTHLH by Lateral-flow immunoassays (LFIAs). The positive results may also point a way to using the same technique for the rapid determination of other relevant cancer proteins.