Cytotoxicity of HBD3 for dendritic cells, normal human epidermal keratinocytes, hTERT keratinocytes, and primary oral gingival epithelial keratinocytes in cell culture conditions
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- 作者：Nattawut Leelakanoka ; nattawut-leelakanok@uiowa.edu" class="auth_mail" title="E-mail the corresponding author ; Carol L. Fischerb ; carol-bratt@uiowa.edu" class="auth_mail" title="E-mail the corresponding author ; Amber M. Batesb ; amber-bates@uiowa.edu" class="auth_mail" title="E-mail the corresponding author ; Janet M. Guthmillerc ; janet.guthmiller@unmc.edu" class="auth_mail" title="E-mail the corresponding author ; Georgia K. Johnsond ; georgia-johnson@uiowa.edu" class="auth_mail" title="E-mail the corresponding author ; Aliasger K. Salema ; aliasger-salem@uiowa.edu" class="auth_mail" title="E-mail the corresponding author ; Kim A. Brogdenb ; d ; kim-brogden@uiowa.edu" class="auth_mail" title="E-mail the corresponding author ; Nicole K. Brogdena ; nicole-brogden@uiowa.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：HBD3 ; Human β-defensin 3 ; DCs ; dendritic cells ; NHEKs ; normal human epidermal keratinocytes ; hTERT ; human telomerase reverse transcriptase keratinocytes ; GE ; gingival epithelial keratinocytes ; PBMCs ; peripheral blood mononuclear cells ; PI ; propidium iodide ; MFI ; median fluorescence intensity ; LD50 ; lethal dose 50
- 刊名：Toxicology Letters
- 出版年：2015
- 出版时间：3 December 2015
- 年：2015
- 卷：239
- 期：2
- 页码：90-96
- 全文大小：1336 K
文摘
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HBD3 is a very versatile host defense peptide.
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It has antimicrobial activities, regulates innate immune mechanisms, and regulates chemokine and cytokine responses.
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HBD3 also has cytotoxicity against eukaryotic cells.
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We show that the cytotoxicity of HBD3 for DCs, NHEKs, hTERT keratinocytes, and GE keratinocytes varied with the cell type and the culture conditions.
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Thus HBD3 has some cytotoxicity, which needs to be considered in future studies of HBD3-modulated chemokine and cytokine responses.