Substances from the filtrate of Phaeodactylum tricornutum were separated by high-performance liquid chromatography (HPLC).
Active compounds from P. tricornutum were screened using activity-guided fraction methods.
Anti-algal compounds from P. tricornutum were isolated and analyzed mainly by HPLC–MS.
The isolated allelochemical was preliminary identified as TYR-PRO-PHE-PRO-GLY-NH2, a type of glycinamides.