Crystal structure and some properties of a major house dust mite allergen, Derf 2

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• 作者：Masashi Suzuki ; Yoshimasa Tanaka ; Seigo Korematsu ; Bunzo Mikami and Nagahiro Minato
• 关键词：Allergen ; House dust mite ; Th shift ; Crystal structure
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2006
• 出版时间：13 January 2006
• 年：2006
• 卷：339
• 期：2
• 页码：679-686
• 全文大小：422 K

文摘

Pyroglyphid house dust mites are a major source of allergens in house dust. Mite allergens sensitize and induce asthma, rhinitis, and eczema in a large portion of patients with allergic diseases. Here, the crystal structure of a major mite allergen, Derf 2, derived from Dermatophagoides farinae was solved by single isomorphous replacement method with anomalous scattering (SIRAS) at 2.1 resolution. The present study also demonstrated that the conformation of the allergen was critical in the determination of Th1/Th2 shift based on physicochemical and immunological analyses. This indicates that rigidly folded and singly dispersed structure is essentially required for the generation of Th2 type cells by the allergen, while conformational variant protein leads to Th1 skewing, irrespective of the same amino acid sequence. This structure/function relationship may allow us to develop a novel strategy for hyposensitization therapy in patients with allergic diseases triggered by house dust mite allergens.