House dust mite major allergen Der f 1 enhances proinflammatory cytokine and chemokine gene expression in a cell line of canine epidermal keratinocytes
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- 作者：Shingo Maeda ; Sadatoshi Maeda ; Sanae Shibata ; Naoki Chimura ; Tsuneo Fukata
- 关键词：Dog ; House dust mite ; Der f 1 ; Inflammatory cytokine ; Atopic dermatitis ; Keratinocyte
- 刊名：Veterinary Immunology and Immunopathology
- 出版年：2009
- 出版时间：15 October 2009
- 年：2009
- 卷：131
- 期：3-4
- 页码：298-302
- 全文大小：209 K
文摘
House dust mite (HDM) allergens are the most common allergens involved in the induction of IgE-mediated hypersensitivity. Recently, epicutaneous sensitization with HDM allergens has been emphasized in the development of atopic dermatitis (AD); however, direct stimulation of canine keratinocytes by mite allergens has not been well investigated. In the present study, we investigated the effects of Der f 1, a major allergen of Dermatophagoides farinae, on cytokine and chemokine gene expression in a canine keratinocyte cell line, CPEK. CPEK constitutively expressed mRNA for TNF-α, IL-12p35, IL-18, GM-CSF, TGF-β, IL-8/CXCL8, TARC/CCL17, CTACK/CCL27 and MEC/CCL28. Of all the cytokines and chemokines investigated in CPEK, transcription levels of GM-CSF, IL-8/CXCL8 and TNF-α mRNA were significantly enhanced by stimulation with Der f 1. The present results suggest that Der f 1 can directly augment inflammatory cytokine and chemokine production from keratinocytes, and may initiate allergic inflammation independently of Type-I hypersensitivity.