Expression of a major house dust mite allergen gene from Dermatophagoides farinae in Lotus japonicus accession miyakojima MG-20

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• 作者：Tomoaki  ; Kato^a ; Yuji  ; Goto^a ; Kazuhisa  ; Ono^b ; Makoto  ; Hayashi^a ; Yoshikatsu  ; Murooka^a ;   ; ^{murooka@bio.eng.osaka-u.ac.jp}
• 关键词：accession Miyakojima MG-20 ; Agrobacterium tumefaciens ; pIG121Hm ; Dermatophagoides farinae ; Der f 1 ; oral allergen-specific immunotherapy
• 刊名：Journal of Bioscience and Bioengineering
• 出版年：2005
• 出版时间：February 2005
• 年：2005
• 卷：99
• 期：2
• 页码：165-168
• 全文大小：393 K

文摘

Transformation of a model legume Lotus japonicus accession Miyakojima MG-20 was examined using Agrobacterium tumefaciens with a binary expression vector. Using the improved transformation method, we introduced a major allergen gene from a house dust mite, Der f 1, into MG-20. Analyses by Southern hybridization, reverse transcription (RT)-PCR, and Western blotting showed that the Der f 1 gene was integrated into the genome of L. japonicus, expressing the gene product in the T1 lines. Our results imply future application of oral allergen-specific immunotherapy using legume plants.