Dendritic cells loaded with apoptotic oligodendrocytes as a source of myelin T-cell epitopes in multiple sclerosis
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- 作者：Francesca Meloni ; Daniele Accapezzato ; Cristina Agresti ; Francesca Aloisi ; Giovanni Ristori ; Marco Salvetti ; Roberto Furlan ; Gianvito Martino ; Vincenzo Barnaba ; Marino Paroli
- 关键词：Multiple sclerosis ; Myelin basic protein ; T lymphocytes ; Apoptosis ; Oligodendrocytes ; Dendritic cells
- 刊名：Clinical Immunology
- 出版年：2008
- 出版时间：November 2008
- 年：2008
- 卷：129
- 期：2
- 页码：286-294
- 全文大小：736 K
文摘
Evidence suggests that T-cell response to myelin basic protein (MBP) plays an important role in multiple sclerosis (MS). However, the mechanism of generation for MBP immunogenic epitopes is unclear. A series of specific CD4+ T-cell lines was obtained by stimulating peripheral blood mononuclear cells from MS patients with synthetic peptides spanning the entire MBP sequence. T-cell lines recognizing MBP(8–27), MBP(13–32), and MBP(23–42) peptides, whose sequences are identical for humans and rats, specifically proliferated and produced large amounts of interferon-γ in response to autologous dendritic cells (DCs) loaded in vitro with apoptotic rat oligodendrocytes. Results suggest that MBP epitopes generated from enzymatic processing of apoptotic glial cells by DCs might be relevant to MS pathogenesis.