Mapping the Landscape of a Eukaryotic Degronome
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- 作者：Yifat Geffen1 ; 4 ; Alon Appleboim1 ; 2 ; 4 ; Richard G. Gardner3 ; Nir Friedman1 ; 2 ; nir@cs.huji.ac.il" class="auth_mail" title="E-mail the corresponding author ; Ronen Sadeh1 ; 2 ; rsadeh@cs.huji.ac.il" class="auth_mail" title="E-mail the corresponding author ; Tommer Ravid1 ; 5 ; tommer.ravid@mail.huji.ac.il" class="auth_mail" title="E-mail the corresponding author
- 刊名：Molecular Cell
- 出版年：2016
- 出版时间：15 September 2016
- 年：2016
- 卷：63
- 期：6
- 页码：1055-1065
- 全文大小：2799 K
文摘
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New reporter-based high-throughput degradation assay was developed
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Thousands of degradation signals (degrons) from the yeast transcriptome were identified
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A secondary screen revealed multiple targets of the E3 ligase Doa10p
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The addition of nuclear localization signal greatly changed degron propensity