Is aristolochic acid nephropathy a widespread problem in developing countries?: A case study of Aristolochia indica L. in Bangladesh using an ethnobotanical-phytochemical approach

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• 作者：Johanna Michl ; Hannah M. Jennings ; Geoffrey C. Kite ; Martin J. Ingrouille ; Monique S.J. Simmonds ; Michael Heinrich
• 关键词：A ; alstonine ; AA ; aristolochic acid ; AAA ; aristolochic acid analogue ; AAN ; aristolochic acid nephropathy ; AL ; aristolactam ; BA ; benzoic acid ; BEN ; Balkan endemic nephropathy ; COSY ; correlation spectroscopy ; CP ; commercial product ; D2O ; deuterated water ; E
• 刊名：Journal of Ethnopharmacology
• 出版年：2013
• 出版时间：26 August, 2013
• 年：2013
• 卷：149
• 期：1
• 页码：235-244
• 全文大小：1586 K

文摘

Ethnopharmacological relevance

Species of Aristolochia are associated with aristolochic acid nephropathy (AAN), a renal interstitial fibrosis and upper urinary tract cancer (UUC). Aristolochic acid nephropathy has been reported in ten countries but its true incidence is unknown and most likely underestimated. By combining an ethnobotanical and phytochemical approach we provide evidence for the risk of AAN occurring in Bangladesh. More specifically, we assess the intra-specific variation of aristolochic acid analogues in medicinally used Aristolochia indica samples from Bangladesh.

Materials and methods

Ethnobotanical information was collected from 16 kavirajes (traditional healers) in different study locations in Bangladesh. Plant samples were obtained from native habitats, botanical gardens, herbal markets and pharmaceutical companies. The samples were extracted using 70 % methanol and were analysed using LC-DAD-MS and ¹H-NMR.

Results

Roots as well as leaves are commonly used for symptoms such as snake bites and sexual problems. Among the informants knowledge about toxicity or side effects is very limited and Aristolochia indica is often administered in very high doses. Replacement of Aristolochia indica with other medicinal plants such as Rauvolfia serpentina (L.) Benth. ex Kurz was common. Aristolochia indica samples contained a variety of aristolochic acid analogues such as aristolochic acid I, aristolochic acid II, cepharadione A and related compounds.

Conclusions

AAN cases are likely to occur in Bangladesh and more awareness needs to be raised about the health risks associated with the use of Aristolochia indica and other species of Aristolochia as herbal medicines.