Marked contribution of alternative end-joining to chromosome-translocation-formation by stochastically induced DNA double-strand-breaks in G2-phase human cells
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- 作者：Aashish Sonia ; Maria Siemanna ; Gabriel E. Panteliasb ; George Iliakisa ; Georg.Iliakis@uk-essen.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：Ionizing radiation (IR) ; Chromosomal translocations ; Classical non homologous end joining (c-NHEJ) ; Alternative end joining (alt-EJ) ; Chromatid translocations
- 刊名：Mutation Research/Genetic Toxicology and Environmental Mutagenesis
- 出版年：2015
- 出版时间：November 2015
- 年：2015
- 卷：793
- 期：Complete
- 页码：2-8
- 全文大小：595 K
文摘
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We investigate the mechanism of translocation formation in human cells.
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We employ ionizing radiation (IR) to randomly induce DSBs in human G2-phase genome.
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Chromosome translocation formation in human cells is largely mediated by alt-EJ.
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Parp and Lig1/Lig3 inhibitors reduce translocations in wt or c-NHEJ deficient cells.
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DSBs by IR and designer nucleases induce translocations by different mechanisms.