Hepatoprotective effect of the ethanol extract of Vitis thunbergii on carbon tetrachloride-induced acute hepatotoxicity in rats through anti-oxidative activities

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• 作者：Jeng-Shyan Deng ; Yi-Chih Chang ; Chi-Luan Wen ; Jung-Chun Liao ; Wen-Chi Hou ; Sakae Amagaya ; Shyh-Shyun Huang ; Guan-Jhong Huang
• 关键词：EVT ; Vitis thunbergii ; ALT ; Alanine aminotransferase ; AST ; aspartate aminotransferase ; SOD ; superoxide dismutase ; CAT ; catalase ; GPx ; glutathione peroxidase ; GSH ; reduced glutathione ; TNF-¦Á ; tumor necrosis factor-alpha ; IL-1 ¦Â ; interleukin-1¦Â ; NO ; nitr
• 刊名：Journal of Ethnopharmacology
• 出版年：2012
• 出版时间：1 August, 2012
• 年：2012
• 卷：142
• 期：3
• 页码：795-803
• 全文大小：987 K

文摘

Ethnopharmacological relevance

Vitis thunbergii var. taiwaniana are traditionally used for the treatment of diarrhea, fracture and injury, jaundice, and hepatitis in Taiwan.

Aim of the study

The hepatoprotective activity of its plant extracts seems to be been associated with its antioxidant activity. This paper aims to investigate the in vitro and in vivo antioxidant effects of the ethanol extract of Vitis thunbergii (EVT).

Materials and methods

In HPLC analysis, the fingerprint chromatogram of EVT was established. Antioxidant ability of EVT was investigated by employing several established in vitro methods. In vivo antioxidant activity was tested against CCl₄-induced toxicity in mice. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were detected in the blood to indicate hepatic injury. Product of lipid peroxidation (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and reduced glutathione (GSH) contents were evaluated for oxidative stress in hepatic injury. Moreover, histopathological observation was assayed for the degree of hepatic injury.

Results

EVT exhibited strong antioxidant ability in vitro. After oral administration of EVT significantly decreased ALT and AST, and ameliorated the oxidative stress in hepatic tissue and increased the activity of CAT, SOD, GPx, and GSH. Serum tumor necrosis factor-alpha (TNF-¦Á), interleukin?1¦Â (IL-1¦Â), and nitric oxide (NO) were decreased in the group treated with CCl₄ plus EVT. Western blotting revealed that EVT blocked protein expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) in CCl₄-treated rats, significantly. Histopathological examination of livers showed that EVT reduced fatty degeneration, cytoplasmic vacuolization and necrosis in CCl₄-treated rats.

Conclusion

This study suggests that EVT possesses antioxidant effects in vitro and hepatoprotective effect on acute liver injuries induced by CCl₄ in vivo, and the results suggested that the effect of EVT against CCl₄-induced liver damage is related to its antioxidant properties.